Identification of a high affinity taurine transporter which is not dependent on chloride |
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Authors: | D B Shennan |
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Institution: | (1) Hannah Research Institute, KA6 5HL Ayr, UK |
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Abstract: | Taurine transport by lactating gerbil mammary tissue has been examined. Taurine uptake is, mediated by a high-affinity system which is specific for -amino acids. The uptake of taurine is Na+-dependent but appears not to be obligatorly dependent upon Cl–. Thus, replacing Na+ with choline almost abolished taurine uptake. Substituting Cl– with NO
3
–
had no effect whereas SCN– induced a small but significant increase in taurine influx. Taurine uptake was Na+-dependent under conditions where Cl– had been replaced with NO
3
–
. However, it is apparent that the Na+-dependent taurine transport system requires the presence of a permeable anion because replacing Cl– with gluconate markedly reduced taurine uptake. Cell-swelling, induced by a hyposmotic challenge, increased the efflux of taurine from gerbil mammary tissue via a pathway sensitive to niflumic acid.Abbreviations Tris
(Tris(hydroxymethyl)aminomethane
- BES
(N,N-bis2-hydroxyethyl]-2-aminoethane sulphonic acid) |
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Keywords: | taurine chloride mammary |
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