Anti-VPg antibody inhibition of the poliovirus replicase reaction and production of covalent complexes of VPg-related proteins and RNA |
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| Authors: | M H Baron D Baltimore |
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| Affiliation: | 1. Whitehead Institute for Biomedical Research Cambridge, Massachusetts 02139 USA;2. Center for Cancer Research and Department of Biology Massachusetts Institute of Technology Cambridge, Massachusetts 02139 USA |
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| Abstract: | Anti-VPg antibodies inhibited host-factor-dependent RNA synthesis by the poliovirus replicase but not oligo(U)-primed synthesis, implicating VPg in the de novo initiation of replicase products. Complexes of VPg-related polypeptide and newly made RNA could be immunoprecipitated by anti-VPg antibody from the host-factor-stimulated products of the replicase reaction. The complexes appeared to be covalently linked and involved 50 to 150 nucleotide chains of RNA that were RNAase-T1-resistant and could be largely poly(U). |
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| Keywords: | Correspondence should be sent to: David J. L. Luck The Rockefeller University 1230 York Avenue New York New York 10021 |
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