| 禾谷缢管蚜体内的病毒结合蛋白基因的克隆与原核表达 |
| |
| 引用本文： | 吴云锋,崔晓锋,林林,周广和.禾谷缢管蚜体内的病毒结合蛋白基因的克隆与原核表达[J].微生物学报,2002,42(4):448-452. |
| |
| 作者姓名： | 吴云锋 崔晓锋 林林 周广和 |
| |
| 作者单位： | 1. 西北农林科技大学,杨凌,712100
2. 中国农业科学院植物保护研究所,北京,100094 |
| |
| 基金项目： | 国家自然科学基金 ( 39970 4 83)，国家八六三青年基金 ( 97 1 37)~~ |
| |
| 摘 要： | 利用一对特异性引物，用PCR的方法从禾谷缢管蚜体内扩增出了病毒结合蛋白基因，序列测定结果表明其长度 为1647 bp，编码548个氨基酸，与GenBank中的禾谷缢管蚜美国生物型Buchnera groELNT核苷酸序列同源性为97%，氨基酸同源性为97.4%。构建了2个原核表达载体并进行表达得到了69kD融合蛋白和63kD的非融合蛋白。
|
| 关 键 词： | 禾谷缢管蚜 内共生细菌 病毒结合蛋白基因 传播 |
| 文章编号： | 0001-6209(2002)04-0448-05 |
| 修稿时间： | 2001年9月3日 |
Cloning and Prokaryotic Expression of Viral Binding Protein(VBP) Gene From Endosybiotic Bacterium of Rhopalosiphum padi |
| |
| Wu Yunfeng,Cui Xiaofeng,Lin Lin,Zhou Guanghe.Cloning and Prokaryotic Expression of Viral Binding Protein(VBP) Gene From Endosybiotic Bacterium of Rhopalosiphum padi[J].Acta Microbiologica Sinica,2002,42(4):448-452. |
| |
| Authors: | Wu Yunfeng Cui Xiaofeng Lin Lin Zhou Guanghe |
| |
| Institution: | Northwest Scie-Tech University of Agriculture and Forestry, Yangling, 712100, China. |
| |
| Abstract: | Viral binding protein gene from Rhopalosiphum padi Yangling biotype was amplified by PCR method and then cloned.The complete nucleotide sequence of the gene was determined,It has 1647 nucleotides encoding 548 amino acids.Comparison showed this gene has 97% identity on nucleotide level with Buchnera groEL AM gene of Rhopalosiphum padi American biotype,while has 97.4% identity on amino acid level was found between this two genes.The VBP gene was ligated into pBV221 and pET30a expression vector and expressed the aim protein 63kD and 69kD. |
| |
| Keywords: | Myzus persicae Endosymbiotic bacterium Virus binding protein Transmission |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《微生物学报》浏览原始摘要信息 |
| 点击此处可从《微生物学报》下载免费的PDF全文 |
