Regulation of nitrogen fixation in Azospirillum brasilense Sp7: Involvement of nifA, glnA and glnB gene products |
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Authors: | Yao Yun Liang,Miklos de,Zamaroczy,Florence Arsè ne,Annick Paquelin,Claudine Elmerich |
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Affiliation: | Département des Biotechnologies, Institut Pasteur, Paris, France. |
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Abstract: | The expression of nifA-, niH- and nifB-lacZ fusions was examined in different mutants of Azospirillum brasilense. Mutations in nifA, glnA and glnB severely impaired the expression of nifH- and nifB-lacZ fusions. By contrast, a nifA-lacZ fusion was not affected in a nifA or a glnB background and was only partially impaired in glnA mutants. It is proposed that in A. brasilense, the PII protein and glutamine synthetase are involved in a post-translational modification of NifA. |
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Keywords: | Azospirillum brasilense Nitrogen fixation regulation Glutamine synthetase P11 protein NifA protein |
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