Enhanced Staining of Bacterial Flagella Using Aged Mordant in the Silver Stain |
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Authors: | Steven M. Finegan Robert A. Smith |
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Affiliation: | a Department of Biological Sciences, Philadelphia College of Pharmacy and Science, Philadelphia, Pennsylvania |
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Abstract: | Intensity of bacterial flagella staining using a modified silver stain was increased by aging the mordant for one week at room temperature. The use of aged mordant increased the apparent diameters of stained flagella and resulted in a darker stain. The mordant remained stable for at least four months at room temperature. The staining protocol presented allows application to liquid or solid cultures. |
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Keywords: | bacteria flagella silver stain mordant |
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