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FCI: an R-based algorithm for evaluating uncertainty of absolute real-time PCR quantification |
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| Authors: | Paolo Verderio Sara Pizzamiglio Fabio Gallo Simon C Ramsden |
| Affiliation: | (1) Unit of Medical Statistics and Biometry, Istituto Nazionale dei Tumori, Milan, Italy;(2) National Genetics Reference Laboratory (Manchester), St. Mary's Hospital, Manchester, UK |
| Abstract: | BackgroundFCI is an R code for analyzing data from real-time PCR experiments. This algorithm estimates standard curve features as well as nucleic acid concentrations and confidence intervals according to Fieller's theorem. |
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