Variability in storage response within a coffee (Coffea spp.) core collection under slow growth conditions |
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Authors: | Stéphane Dussert Nathalie Chabrillange François Anthony Florent Engelmann Christine Recalt Serge Hamon |
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Institution: | (1) ORSTOM, Laboratoire des Ressources Génétiques et d'Amélioration des Plantes Tropicales, BP 5045, F-34032 Montpellier Cedex 1, France;(2) CATIE, Apartado 59, 7170 Turrialba, Costa Rica;(3) IPGRI, Via delle Sette Chiese 142, I-00145 Rome, Italy |
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Abstract: | Anin vitro core collection of African coffee germplasm, structured in 32 diploid diversity groups, was established and conserved under slow growth for 3 years (6 subcultures). The initial objective was to store twenty accessions per group, with four replicates per accession. A statistical model was developed to analyse observations of survival rates within each diversity group. The goodness of fit of the model was shown. Survival analysis indicated a broad variability of the accessions in their response to the storage conditions and confirmed the importance of structuring the coffee complex down to the intraspecific level. Intra- and inter-group differences had consequences on the genetic representativity of thein vitro core collection. For practical purposes, conservation was carried on when the intra-group genetic drift was less than 50%.Abbreviations BA
6-benzyladenine
- CAR
Central African Republic
- CIRAD
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
- FAO
Food and Agriculture Organization
- IBPGR
International Board for Plant Genetic Resources
- IDEFOR-DCC
Institut Des Fôrets - Département Café Cacao
- ORSTOM
Institut français de recherche scientifique pour le développement en coopération |
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Keywords: | Coffea in vitro storage survival model genetic resources |
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