Characterization and cross-species amplification of microsatellites from the endangered Hawksbill turtle (Eretmochelys imbricate) |
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Authors: | Grace Lin Alex Chang Hon Wah Yap Gen Hua Yue |
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Institution: | (1) Molecular Population Genetics Group, Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore, 117604, Singapore;(2) Reproductive Genomics Group, Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore, 117604, Singapore;(3) Underwater World Singapore, Singapore, Singapore |
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Abstract: | Twelve novel polymorphic microsatellites were isolated from the endangered Hawksbill turtle (Eretmochelys imbricate). Eight of 12 markers were used to study genetic diversity of two sea turtle species: E. imbricate and green sea turtle (C. mydas). In E. imbricate, the average allele number of the eight microsatellites was 6.25/locus with a range of 3–13. The average expected and observed
heterozygosity was 0.66 and 0.63 respectively. In C. mydas, the average allele number of the eight markers was 11.63/locus. The observed heterozyosity (0.68) was lower than the expected
heterozyosity (0.79). Most of 12 microsatellites amplified specific and polymorphic PCR products in other six turtle species.
Hence, the developed microsatellites would facilitate studies on genetic diversity and population structure of E. imbricate and other marine turtle species. |
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Keywords: | Hawksbill turtle Microsatellite Diversity Conservation |
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