Enzymatic digestion of spent yeast cells for nutrient recycling in inulase production |
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Authors: | Kin Sing Lam J. W. D. GrootWassink |
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Affiliation: | (1) Dept. 203, Bristol-Myers Squibb Company, P.O. Box 5100, 06492 Wallingford, CT, USA;(2) Plant Biotechnology Institute, National Research Council, Saskatoon, Saskatchewan, Canada |
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Abstract: | Summary An enzyme complex capable of lysing yeast cells was produced byArthrobacter sp. in a medium containing live cells ofKluyveromyces fragilis as the sole source of nutrients. The enzyme complex caused a 90% reduction in the optical density of viable yeast cells in 6 h at 25°C. This yeast cell hydrolysate can be used as a source of nitrogen, vitamins and minerals for subsequent growth of yeast cells (8.3 mg/ml) and further production of inulase (167 U/ml) representing 88 and 87% yield respectively, compared to cells grown on a standard yeast extract (1%) and sucrose (2%) medium. |
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Keywords: | Kluyveromyces fragilis Arthrobacter Yeast lytic enzyme complex |
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