Application of several types of substrates to ficin-catalyzed peptide synthesis |
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| Authors: | H. Sekizaki E. Toyota T. Fuchise S. Zhou Y. Noguchi K. Horita |
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| Affiliation: | (1) Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Hokkaido, Japan;(2) Pharmic Department, Medical College, Qingdao University, Qingdao, China |
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| Abstract: | Summary. The capability of ficin, a cystine protease, to form peptide bonds was investigated using several types of N-Boc-amino acid phenyl and naphthyl esters as acyl donor components. Enzyme-catalyzed peptide synthesis was carried out under optimized reaction conditions of pH, acyl acceptor concentration and selection of the best yield organic solvent. It used a condensation of N-Boc-Ala-OpGu and Ala-pNA as a model reaction. The products were obtained in 72–96% yield using 10 different substrates, within a few minutes of reaction time. Authors’ address: Prof. Haruo Sekizaki, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, Japan |
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| Keywords: | : N-Boc-Amino acid – Amidinophenyl esters – Guanidinophenyl esters – (Guanidinomethyl)phenyl ester – Guanidinonaphthyl ester – Enzymatic peptide synthesis – Ficin |
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