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慢性缺氧的猪肺动脉内皮细胞在急性缺氧时PDGF-B链mRNA的表达
引用本文:孔炜,叶仕桥,王迪浔,孙秉庸.慢性缺氧的猪肺动脉内皮细胞在急性缺氧时PDGF-B链mRNA的表达[J].中国组织化学与细胞化学杂志,2001,10(3):259-262.
作者姓名:孔炜  叶仕桥  王迪浔  孙秉庸
作者单位:1. 华中科技大学同济医学院病理生理学教研室
2. 第三军医大学
基金项目:国家自然科学基金重点项目资助课题 (No.39730190)
摘    要:采用原位杂交技术结合图象分析检测常氧(PO2±21.3kPa)及慢性缺氧(P025.3±0.7kPa)培养的猪肺动脉内皮细胞PDGF-BmRNA的表达及其对急性缺氧刺激的反应.结果常氧及慢性缺氧培养的肺动脉内皮细胞(PAEC)在急性缺氧后PDGF-BmRNA表达均增加(P<0.05),以第4、6代慢性缺氧组升高的幅度更大.结果表明慢性缺氧可增强PAEC在急性缺氧时PDGF-BmRNA的表达,可能促进肺血管改建和肺动脉高压的发展.

关 键 词:慢性缺氧    肺动脉内皮细胞  急性缺氧  PDGF-B链  mRNA  表达  血小板源性生长因子
修稿时间:2000年11月1日

THE EXPRESSION OF PDGF-B mRNA IN RESPONSE TO ACUTE HYPOXIA IN P ORCINEPULMONARY ARTERY ENDOTHELIAL CELLS CULTURED UNDER CHRONIC HYPOXIA
Kong Wei,Ye Shiqiao,Wang Dixun,Sun Bingyong.THE EXPRESSION OF PDGF-B mRNA IN RESPONSE TO ACUTE HYPOXIA IN P ORCINEPULMONARY ARTERY ENDOTHELIAL CELLS CULTURED UNDER CHRONIC HYPOXIA[J].Chinese Journal of Histochemistry and Cytochemistry,2001,10(3):259-262.
Authors:Kong Wei  Ye Shiqiao  Wang Dixun  Sun Bingyong
Abstract:In situ hybridization and image analysis system were used to investigate the expression of PDGF B mRNA in porcine pulmonary artery endothelial cells (PAEC) cultured under normoxia and chronic hypoxia conditions. The different responses to acute hypoxia were evaluated. Results: Acute hypoxia enhanced the expression of PDGF B mRNA in both the normoxic and chronic hypoxic groups. the increase being more obvious in the latter group. Conclusion: Chronic hypoxia can increase the expression of PDGF B mRNA in response to acute hypoxia in PAEC, which may contribute to the remodeling of pulmonary arteries and the development of pulmonary hypertension.
Keywords:Hypoxia  Pulmonary artery  Endothelial cells  Platelet  derived growth factors  Porcine
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