| 慢性缺氧的猪肺动脉内皮细胞在急性缺氧时PDGF-B链mRNA的表达 |
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| 引用本文： | 孔炜,叶仕桥,王迪浔,孙秉庸.慢性缺氧的猪肺动脉内皮细胞在急性缺氧时PDGF-B链mRNA的表达[J].中国组织化学与细胞化学杂志,2001,10(3):259-262. |
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| 作者姓名： | 孔炜 叶仕桥 王迪浔 孙秉庸 |
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| 作者单位： | 1. 华中科技大学同济医学院病理生理学教研室
2. 第三军医大学 |
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| 基金项目： | 国家自然科学基金重点项目资助课题 (No.39730190) |
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| 摘 要： | 采用原位杂交技术结合图象分析检测常氧(PO2±21.3kPa)及慢性缺氧(P025.3±0.7kPa)培养的猪肺动脉内皮细胞PDGF-BmRNA的表达及其对急性缺氧刺激的反应.结果常氧及慢性缺氧培养的肺动脉内皮细胞(PAEC)在急性缺氧后PDGF-BmRNA表达均增加(P＜0.05),以第4、6代慢性缺氧组升高的幅度更大.结果表明慢性缺氧可增强PAEC在急性缺氧时PDGF-BmRNA的表达,可能促进肺血管改建和肺动脉高压的发展.
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| 关 键 词： | 慢性缺氧 猪 肺动脉内皮细胞 急性缺氧 PDGF－B链 mRNA 表达 血小板源性生长因子 |
| 修稿时间： | 2000年11月1日 |
THE EXPRESSION OF PDGF-B mRNA IN RESPONSE TO ACUTE HYPOXIA IN P ORCINEPULMONARY ARTERY ENDOTHELIAL CELLS CULTURED UNDER CHRONIC HYPOXIA |
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| Kong Wei,Ye Shiqiao,Wang Dixun,Sun Bingyong.THE EXPRESSION OF PDGF-B mRNA IN RESPONSE TO ACUTE HYPOXIA IN P ORCINEPULMONARY ARTERY ENDOTHELIAL CELLS CULTURED UNDER CHRONIC HYPOXIA[J].Chinese Journal of Histochemistry and Cytochemistry,2001,10(3):259-262. |
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| Authors: | Kong Wei Ye Shiqiao Wang Dixun Sun Bingyong |
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| Abstract: | In situ hybridization and image analysis system were used to investigate the expression of PDGF B mRNA in porcine pulmonary artery endothelial cells (PAEC) cultured under normoxia and chronic hypoxia conditions. The different responses to acute hypoxia were evaluated. Results: Acute hypoxia enhanced the expression of PDGF B mRNA in both the normoxic and chronic hypoxic groups. the increase being more obvious in the latter group. Conclusion: Chronic hypoxia can increase the expression of PDGF B mRNA in response to acute hypoxia in PAEC, which may contribute to the remodeling of pulmonary arteries and the development of pulmonary hypertension. |
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| Keywords: | Hypoxia Pulmonary artery Endothelial cells Platelet derived growth factors Porcine |
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