NIDDM发病前后中国地鼠胰岛和肝脏细胞GLUT的变化研究

用胶原酶法分离胰岛细胞、超速离心法提取中国地鼠胰岛和肝脏细胞膜蛋白，ＳＤＳ电泳、ＷｅｓｔｅｒｎＢｌｏｔｔｉｎｇ分离ＧＬＵＴ后，应用ＧＬＵＴ２抗体免疫结合法测定ＧＬＵＴ的含量与分子量，同时测定了动物血糖、尿糖和血浆胰岛素的含量、显微镜下直接计数了胰岛细胞总数。结果表明：ＮＩＤＤＭ发病后，自发ＮＩＤＤＭ中国地鼠胰岛细胞ＧＬＵＴ含量减少，与ＧＬＵＴ２抗体反应明显减弱，但肝脏ＧＬＵＴ无明显变化。胰岛细胞总数发病前后大体一致。而血糖、尿精及血浆胰岛素浓度明显升高。

Variations of GLUT in NIDDM Chinese Hamsterpancreatic islets and liver tissue

fter the pancreatic islets were isolated from the Chinese Hamster bycollagenase digestion, the membrance of the islets and liver cells wereprepared by ultracentrifuge, gel electrophoresis and Western blotting. TheGLUT were determined by immunoreaction and the unine glucose concentra-tions of the serum glucose, insulin and the urine glucose were also determined in the same experiment. The results showed that after pathogenesisof the NIDDM, the quantity of GLUT in pancreatic islets were signifi-cantly reduced and the immunoreaction of the GLUT with antibodieswere also weakened.However, there are no significant difference inthe liver GLUT and in the members of the pancreas islets GLUT.But theconcentrations of glucose in urine and serum and the serum insulin weresignificantly increased after pathogenesis of the NIDDM in Chinese Ham-ster .