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Production and Characterization of Monoclonal Antibodies of Shrimp White Spot Syndrome Virus Envelope Protein VP28 |
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| Authors: | Wan-gang GU Jun-fa YUAN Ge-lin XU Li-juan LI Ni LIU Cong ZHANG Jian-hong ZHANG Zheng-li SHI |
| Affiliation: | [1]State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China [2]Wuhan Institute of Biological Products, Ministry of Health, Wuhan 430060, China [3]College of Life Science, Central China Normal University, Wuhan 430079, China |
| Abstract: | BALB/c mice were immunized with purified White spot syndrome virus (WSSV). Six monoclonal antibody cell lines were selected by ELISA with VP28 protein expressed in E. coli. in vitro neutralization experiments showed that 4 of them could inhibit the virus infection in crayfish. Western-blot suggested that all these monoclonal antibodies were against the conformational structure of VP28. The monoclonal antibody 7B4 was labeled with colloidal gold particles and used to locate the VP28 on virus envelope by immunogold labeling. These monoclonal antibodies could be used to develop immun-ological diagnosis methods for WSSV infection. |
| Keywords: | White spot syndrome virus (WSSV) Mab Envelope Localization Neutralization |
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