In Vivo imaging of differences in early donor cell proliferation in graft-versus-host disease hosts with different pre-conditioning doses |
| |
Authors: | Myung Geun Song Bora Kang Ji Yeong Jeon Jun Chang Seungbok Lee Chang-Ki Min Hyewon Youn Eun Young Choi |
| |
Institution: | Department of Tumor Biology, Seoul National University College of Medicine, Seoul 110-799, Korea. |
| |
Abstract: | Graft-versus-host disease (GVHD) results from immunemediated attacks on recipient tissues by donor-originated cells through
the recognition of incompatible antigens expressed on host cells. The pre-conditioning irradiation dose is a risk factor influencing
GVHD severity. In this study, using newly generated luciferase transgenic mice on a B6 background (B6.LucTg) as bone marrow and splenocyte donors, we explored the effects of irradiation doses on donor cell dynamics in major histocompatibility
complex (MHC)-matched allogeneic GVHD hosts via bioluminescence imaging (BLI). Results from BLI of GVHD hosts showed higher
emission intensities of luminescence signals from hosts irradiated with 900 cGy as compared with those irradiated with 400
cGy. In particular, BLI signals from target organs, such as the spleen, liver, and lung, and several different lymph nodes
fluctuated with similar time kinetics soon after transplantation, reflecting the synchronous proliferation of donor cells
in the different organs in hosts irradiated with 900 cGy. The kinetic curves of the BLI signals were not synchronized between
the target organs and the secondary organs in hosts irradiated with 400 cGy. These results demonstrate that pre-conditioning
doses influence the kinetics and degree of proliferation in the target organs soon after transplantation. The results from
this study are the first describing donor cell dynamics in MHC-matched allogeneic GVHD hosts and the influence of irradiation
doses on proliferation dynamics, and will provide spatiotemporal information to help understand GVHD pathophysiology. |
| |
Keywords: | bioluminescence imaging graft-versus-host disease irradiation dose luciferase transgenic mouse |
本文献已被 PubMed SpringerLink 等数据库收录! |
|