Regeneration and characterization of plants obtained from anther cultures in Medicago sativa L |
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Authors: | Nedyalka Zagorska Boyan Dimitrov Polina Gadeva Penka Robeva |
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Institution: | (1) Institute of Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria |
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Abstract: | Summary The androgenic ability of four Medicago sativa L. genotype (Boynitza 5, Byala, 494, and 3815) was tested. Callus and organogenesis were induced in all lines studied. The
percentage of anthers producing calluses and organogenesis showed wide variation (calluses—from 11% up to 77%; organogenesis—4.8%
to 15.2%). It has been established that genotype, nutrient medium composition, and stage of pollen development considerably
affected both callus production and organogenesis. Androgenesis in M. sativa could be achieved via callus and direct embryogenesis. About 500 morphologically different regenerants were obtained. Wide
variability in chromosome number of regenerated plants was observed by cytological studies. Haploid, dihaploid, as well as
mixoploid plants were obtained. |
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Keywords: | Medicago sativa L haploid anther culture androgenesis callus organogenesis |
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