Selection and use of SNP markers for animal identification and paternity analysis in U.S. beef cattle |
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Authors: | Michael P Heaton Gregory P Harhay Gary L Bennett Roger T Stone W Michael Grosse Eduardo Casas John W Keele Timothy PL Smith Carol G Chitko-McKown William W Laegreid |
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Institution: | (1) USDA, ARS, U.S. Meat Animal Research Center (MARC), State Spur 18D, P.O. Box 166, Clay Center, Nebraska 68933-0166, USA, US |
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Abstract: | DNA marker technology represents a promising means for determining the genetic identity and kinship of an animal. Compared
with other types of DNA markers, single nucleotide polymorphisms (SNPs) are attractive because they are abundant, genetically
stable, and amenable to high-throughput automated analysis. In cattle, the challenge has been to identify a minimal set of
SNPs with sufficient power for use in a variety of popular breeds and crossbred populations. This report describes a set of
32 highly informative SNP markers distributed among 18 autosomes and both sex chromosomes. Informativity of these SNPs in
U.S. beef cattle populations was estimated from the distribution of allele and genotype frequencies in two panels: one consisting
of 96 purebred sires representing 17 popular breeds, and another with 154 purebred American Angus from six herds in four Midwestern
states. Based on frequency data from these panels, the estimated probability that two randomly selected, unrelated individuals
will possess identical genotypes for all 32 loci was 2.0 × 10−13 for multi-breed composite populations and 1.9 × 10−10 for purebred Angus populations. The probability that a randomly chosen candidate sire will be excluded from paternity was
estimated to be 99.9% and 99.4% for the same respective populations. The DNA immediately surrounding the 32 target SNPs was
sequenced in the 96 sires of the multi-breed panel and found to contain an additional 183 polymorphic sites. Knowledge of
these additional sites, together with the 32 target SNPs, allows the design of robust, accurate genotype assays on a variety
of high-throughput SNP genotyping platforms. |
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