Molecular cloning of theEscherichia
coli gene encoding xylose isomerase |
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Authors: | P P Ueng K J Volpp J V Tucker C S Gong L F Chen |
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Institution: | (1) Department of Food Science, Purdue University, 47907 West Lafayette, IN |
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Abstract: | Summary A 2.4 Kb DNA fragment restricted from a Clarke-Carbon ColEl plasmid, pLC32-9, containing the xylose isomerase gene has been inserted into the PstI site of pDB248, a shuttle plasmid between the bacteriumE.
coli and the fission yeast,Schizosaccharomyces
pombe. This recombinant plasmid, pDB248-XI, can genetically complement xylose isomerase deficientE.
coli strains and xylose isomerase gene can be expressed inSchizosaccharomyces
pombe. |
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Keywords: | |
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