Participation of the hup gene product in site-specific DNA inversion in Escherichia coli |
| |
| Authors: | M Wada K Kutsukake T Komano F Imamoto Y Kano |
| |
| Affiliation: | Laboratory of Molecular Genetics, Riken, Tsukuba Life Science Center, Ibaraki, Japan. |
| |
| Abstract: | The closely related Escherichia coli genes hupA and hupB each encode a bacterial histone-like protein HU. We report here that DNA inversion mediated by hin, gin, pin and rci but not by cin is blocked in a hupA hupB double mutant, although inversions in these systems occur in the hupA or hupB single mutant as efficiently as in the wild-type strain. These findings show that HU protein participates in site-specific DNA inversion in E. coli and that only one subunit, either HU-1 or HU-2, is sufficient for this inversion. |
| |
| Keywords: | |
|
|
