Drug resistance marker-aided genome shuffling to improve acetic acid tolerance in <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis> |
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Authors: | Dao-Qiong Zheng Xue-Chang Wu Pin-Mei Wang Xiao-Qin Chi Xiang-Lin Tao Ping Li Xin-Hang Jiang Yu-Hua Zhao |
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Institution: | (1) Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, People’s Republic of China; |
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Abstract: | Acetic acid existing in a culture medium is one of the most limiting constraints in yeast growth and viability during ethanol
fermentation. To improve acetic acid tolerance in Saccharomyces cerevisiae strains, a drug resistance marker-aided genome shuffling approach with higher screen efficiency of shuffled mutants was developed
in this work. Through two rounds of genome shuffling of ultraviolet mutants derived from the original strain 308, we obtained
a shuffled strain YZ2, which shows significantly faster growth and higher cell viability under acetic acid stress. Ethanol
production of YZ2 (within 60 h) was 21.6% higher than that of 308 when 0.5% (v/v) acetic acid was added to fermentation medium.
Membrane integrity, higher in vivo activity of the H+-ATPase, and lower oxidative damage after acetic acid treatment are the possible reasons for the acetic acid-tolerance phenotype
of YZ2. These results indicated that this novel genome shuffling approach is powerful to rapidly improve the complex traits
of industrial yeast strains. |
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