Improved primer sequences for the mitochondrial ND1, ND3/4 and ND5/6 segments in salmonid fishes: application to RFLP analysis of Atlantic salmon |
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| Authors: | E E Nielsen ‡ M M Hansen † K-L D Mensberg † |
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| Institution: | Department of Ecology and Genetics, University of Aarhus, Ny Munkegade, Building 540, 8000 Aarhus C. Denmark;Danish Institute for Fisheries Research, Department of Inland Fisheries, Vejlsøvej 39, 8600 Silkeborg, Denmark |
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| Abstract: | New specific primers for the mtDNA segments ND1, ND3/4 and ND5/6 designed from the rainbow trout sequence, improved PCR amplification for salmonid fishes. RFLP analysis revealed restriction site variation for all three segments in Atlantic salmon. Eleven haplotypes were detected in a screening of 30 individuals from four European populations. |
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| Keywords: | Salmo salar mtDNA primers RFLP analysis |
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