Photolabeling of staphylococcal alpha-toxin from within rabbit erythrocyte membranes |
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| Authors: | M Thelestam C Jolivet-Reynaud J E Alouf |
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| Institution: | 1. Department of Bacteriology, Karolinska institutet, S-104 01 Stokholm, Sweden;2. Unité des Antigènes Bacteriens, (Equipe de Recherche associée au C.N.R.S. No 794), Institut Pasteur, 28 Rue du Docteur Roux, 757 24 Paris, Cedex 15, France |
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| Abstract: | Intrinsic membrane proteins of rabbit red blood cells were labeled with the photoreactive amphipatic reagent 12-(4-azido-2-nitrophenoxy) stearoyl (1-14C) glucosamine, which inserts into the hydrophobic membrane region and generates a reactive nitrene upon ultraviolet irradiation. Photolabeling of membrane-bound staphylococcal alpha-toxin after lysis of probe-treated rabbit red blood cells by this toxin implies its penetration into the hydrophobic region of the outer leaflet of the membrane. In contrast clostridial theta-toxin and staphylococcal delta-toxin were not labeled, but extraction of intrinsic membrane proteins by delta-toxin was evidenced. |
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| Keywords: | Rabbit red blood cells RRBC 12-APS-GlcN phosphate buffered saline PBS |
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