Occurrence of <Emphasis Type="Italic">Xanthomonas axonopodis</Emphasis> pv. <Emphasis Type="Italic">phaseoli</Emphasis>, the causal agent of common bacterial blight disease,on seeds of common bean (<Emphasis Type="Italic">Phaseolus vulgaris</Emphasis> L.) in upper Egypt |
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Authors: | M H Abd-Alla S R Bashandy S Schnell |
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Institution: | 1.Botany Department, Faculty of Science,Assuit University,Assuit,Egypt;2.Institute of Applied Microbiology, Interdisciplinary Research Center (IFZ),Justus-Liebig-Universit?t Giessen,Giessen,Germany |
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Abstract: | Common bean seed lots collected from different seed dealers and Malawii agriculture station were screened for the presence
of Xanthomonas axonopodis pv. phaseoli. In the laboratory the pathogen was isolated following the routine laboratory assay method, i.e. direct plating method using yeast extract-dextrose-calcium carbonate agar medium (YDC). Yellow, convex, mucoid colonies of
Xanthomonas were consistently isolated on YDC from seed samples. The presumptive pathogen was confirmed by isolation on semiselective
medium, such as mTBM and MD5A. Further, the pathogen was confirmed by biochemical, physiological and, finally, the pathogenicity
tests. Five samples out of seven were positive for Xanthomonas. The isolates were found to cause common blight of 3-week-old common bean plants by 7 d after inoculation. Bacteria with
the same characteristics as those inoculated were re-isolated from the infected plants. |
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