利用酶催化反应过程曲线确定动力学参数的新方法

本文提出了一个利用过程曲线确定酶催化反应动力学参数的新方法.利用这一方法,仅仅根据两条实验曲线就可以确定单底物酶催化反应的全部动力学参数,并且所有的图形都是(?)

AN ALTERNATIVE PLOTTING METHOD USING PROGRESS CURVES OF ENZYME REACTION TO DETERMINE KINETIC PARAMETERS

A plotting method has been developed to determine kinetic parmeters by using progress curves of enzyme reaction. The method has two are antages: 1. All kinetic constants, Km, Vmax , Kp and Kp, can be obtained in only two experiments; 2. All plots are linear which may provide a more reliable estimation for the reaction mechanisms and the kinetic constants. In addition, this method is also applicable to the cases of substrate inhibition and simple two - substrate. enzyme reaction, and hence it may be useful in study on the kinetics of enzyme - catalyzed reaction.