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Suppression for the proliferation of fibroblasts by external DNA |
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| Authors: | Hidemitsu Kitamura Nobuko Takemoto Morimichi Mizuno Yoshinori Kuboki Nobuo Sakairi Norio Nishi |
| Institution: | a Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060, Japan b Department of Biochemistry, School of Dentistry, Hokkaido University, Kita-ku, Sapporo 060, Japan |
| Abstract: | Phase-contrast and fluorescence microscopic observation showed that DNA added in the cell-culture medium for fibroblasts localized just on the surface of fibroblasts. The DNA bound to fibroblasts was found to be eluted by treating with collagenase. The suppression for the proliferation of fibroblasts by external DNA was confirmed with microscopic observation for the cells cultured in the presence and absence of DNA. Proliferation of the cells decreased from 412 to 155% by the addition of DNA. These results indicate that DNA has an affinity for collagen, the most major extracellular-matrix produced by fibroblasts, and suppresses the growth of fibroblasts. |
| Keywords: | DNA Fibroblasts Collagen Extracellular matrix Growth inhibitor |
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