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An Indirect ELISA of Classical Swine Fever Virus Based on Quadruple Antigenic Epitope Peptide Expressed in E.coli |
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| Authors: | Guo-zhen LIN Fu-ying ZHENG Ji-zhang ZHOU Xiao-an CAO Xiao-wei GONG Guang-hua WANG Chang-qing QIU |
| Affiliation: | Guo-zhen LIN,Fu-ying ZHENG,Ji-zhang ZHOU,Xiao-an CAO,Xiao-wei GONG,Guang-hua WANG , Chang-qing QIU ** (Key Laboratories of Grazing Animal Diseases , Animal Virology,Ministry of Agriculture,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China,State Key Laboratory of Veterinary Etiological Biology,China) |
| Abstract: | In this study,a synthesized quadruple antigenic epitope gene region of the classical swine fever virus (CSFV)E2 glycoprotein was expressed in E.coli to a obtain target protein.This target protein was used as a coating antigen to establish an indirect ELISA for specifically detecting anti-CSFV antibodies in serum samples from pigs.The P/N cut-off value of this assay was 1.92 by receiver operating characteristic curve(ROC)analysis based on 30 negative sera and 80 positive samples.The test gave 97.5%sensitivit... |
| Keywords: | Antigenic epitope Bovine viral diarrhoea virus(BVDV) Classical swine fever virus(CSFV) Expression Indirect ELISA |
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