Expression ofBordetella pertussis toxin subunits inBacillus subtilis |
| |
| Authors: | Per E. J. Saris Ulla Airaksinen Satu Nurmiharju Kate Runeberg-Nyman Ilkka Palva |
| |
| Affiliation: | (1) Genesit Oy, Valimotie 7 D, SF-00380 Helsinki, Finland;(2) Molecular Biology Unit, National Public Health Institute, Mannerheimintie 166, SF-0030 Helsinki, Finland |
| |
| Abstract: | Summary Pertussis toxin subunits (S1–S5) were expressed inBacillus subtilis using a vector with the promoter, Shine-Dalgarno sequence and the sequence coding for the first 7 amino acids of the signal sequence of the  -amylase gene fromB. amyloliquefaciens. The use of this vector resulted in a high level of intracellular expression of each pertussis toxin subunit as aggregates in the cytoplasm ofB. subtilis. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
