Uptake, Distribution, and Metabolism of 1-Naphthaleneacetic Acid and Indole-3-Acetic Acid During Callus Initiation From Actinidia deliciosa Tissues |
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Authors: | M L Centeno B Fernández I Feito A Rodríguez |
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Institution: | (1) Departamento de Biología Vegetal, Universidad de Santiago de Compostela, E.P.S., Campus de Lugo, 27002 Lugo, Spain, ES;(2) Laboratorio de Fisiología Vegetal, Dpto. B.O.S., Universidad de Oviedo. C/Catedrático Rodrigo Uría, s/n. 33071-Oviedo, Spain, ES;(3) Instituto Universitario de Biotecnología de Asturias (Asociado al CSIC), 33071-Oviedo, Spain, ES |
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Abstract: | 1-Naphthaleneacetic acid (NAA) and 6-benzyladenine (BA) were required for in vitro callus formation at the basal edge of
kiwifruit (Actinidia deliciosa A. Chev] Liang and Ferguson, cv. Hayward) petioles. The uptake, metabolism, and concentration of NAA and indole-3-acetic
acid (IAA) content were examined in the explants during the callus initiation period. After 1, 6, 12, 24, 48, and 96 h of
culture in the presence of H3]NAA, petioles were divided into apical, middle, and basal portions and analyzed. Except for a high IAA level measured at
12 h, IAA content decreased in tissues during a culture period of 96 h. NAA uptake was higher in petiolar edges than in the
middle portion, and NAA was rapidly conjugated with sugars and aspartic acid inside the tissues. The amide conjugation was
triggered in apical and basal portions from 12 h and in the middle part from 48 h, with α-naphthylacetylaspartic acid being
the major metabolite. Free-NAA concentration in cultured petioles achieved an equilibrium with the exogenously applied NAA
(0.27 μm) from 12 h, and it remained constant thereafter. The relationships between the role attributed to NAA and BA in the initiation
and the maintenance of disorganized growth of callus in kiwifruit cultures are discussed.
Received December 21, 1998; accepted July 20, 1999 |
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Keywords: | ,Actinidia deliciosa—,Auxin—,Callus—,Indoleacetic acid—,Kiwifruit—,Naphthaleneacetic acid |
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