Differences in cell wall polysaccharide composition between embryogenic and non-embryogenic calli of <Emphasis Type="Italic">Medicago arborea</Emphasis> L. |
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| Authors: | Vanessa Endress Jorge Barriuso Pilar Ruperez Juan Pedro Martin Antonio Blazquez Nieves Villalobos Hilario Guerra Luisa Martin |
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| Institution: | 1.Departamento de Biología Vegetal, Facultad de Biología,Universidad Complutense de Madrid,Madrid,Spain;2.Departamento de Ciencias Ambientales y Recursos Naturales, Facultad de Farmacia,Universidad CEU San Pablo,Madrid,Spain;3.Departamento de Metabolismo y Nutrición,Instituto del Frio, CSIC,Madrid,Spain;4.Departamento de Fisiología Vegetal, Facultades de Biología y Farmacia,Universidad de Salamanca,Salamanca,Spain;5.Departamento de Biología Vegetal, Escuela Técnica Superior de Ingenieros Agrónomos,Universidad Politécnica de Madrid,Madrid,Spain;6.Departamento de Estadística, Facultad de Economía y Empresa,Universidad de Salamanca,Salamanca,Spain |
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| Abstract: | Analysis of cell wall polysaccharide composition of embryogenic and non-embryogenic calli obtained from hypocotyl and petiole
explants from Medicago arborea L. revealed significant differences. For calli induced from both hypocotyls and petioles, levels of total sugars, pectins,
and hemicelluloses were higher in embryogenic than in non-embryogenic calli. Whereas in the residual cellulose fraction, the
highest levels of sugar were detected in non-embryogenic calli. When comparing the two donor sources of callus explants, the
highest total sugar levels were detected in embryogenic calli induced from petioles, mainly in the pectin fraction and to
a lesser extent in the hemicellulose fraction. Moreover, analysis of uronic acids revealed higher levels in embryogenic calli,
primarily in the pectin fraction. Analysis of those sugars associated with cell walls of calli suggested that these polysaccharides
consisted of pectic polysaccharides and glucans, and that their levels were higher in embryogenic than non-embryogenic calli. |
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| Keywords: | Cell wall Embryogenic calli Medicago arborea L subsp arborea Non-embryogenic calli Sugars Uronic acids |
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