A rise in cytosolic calcium is not necessary for maturation of Xenopus laevis oocytes |
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| Authors: | R J Cork M F Cicirelli K R Robinson |
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| Affiliation: | 1. Division of Cardiology, Rocky Mountain Regional VA Medical Center, University of Colorado, Denver, CO, USA;2. Division of Cardiovascular Medicine, University of California, Davis, CA, USA;1. Robinson Research Institute, University of Adelaide, Australia;2. Center for Reproductive Medicine, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine Shanghai, China;1. Department of Gynaecology and Obstetrics of the First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong Province 515041, People''s Republic of China;2. Laboratory of Cell Senescence, Shantou University Medical College, 22 Xin Ling Rd, Jinping District, Shantou, Guangdong Province 515041, People''s Republic of China;3. Department of Gynaecology, Obstetrics of Shantou Municipal Central Hospital, Shantou, Guangdong Province 515041, People''s Republic of China |
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| Abstract: | Cytoplasmic free calcium levels during progesterone-induced meiotic maturation in Xenopus laevis oocytes were measured using the photoprotein aequorin. The resting level of [Ca2+]i was 92.6 +/- 30 nM. No significant changes were observed after progesterone addition, although a large pulse of [Ca2+]i was observed upon activation of matured oocytes. These findings are discussed in terms of the role of calcium in maturation and it is concluded that calcium is not the second messenger for progesterone. This conclusion is further supported by the finding that 100 microM TMB-8, a blocker of intracellular calcium release, had no effect on progesterone-induced maturation. |
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