Cell wall polymers of Actinomadura carminata INA 4281 |
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Authors: | I. B. Naumova N. V. Potekhina C. Duigimbaye A. S. Shashkov L. P. Terekhova T. P. Preobrazhenskaya |
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Affiliation: | (1) Department of Biology, Moscow State University, 119899 Moscow, USSR;(2) N. D. Zelinsky Institute of Organic Chemistry, USSR Academy of Medical Sciences, Moscow State University, 119899 Moscow, USSR;(3) Institute for New Antibiotics, USSR Academy of Medical Sciences, Moscow State University, 119899 Moscow, USSR |
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Abstract: | The cell walls of Actinomadura carminata INA 4281 were found to contain peptidoglycan, teichoic acid, and nonpeptidoglycan amino acids. The peptidoglycan was of the A1 type and contained a small amount of ll-DAP in addition to m-DAP. The teichoic acid was an 1,3-poly(glycerol phosphate) chain composed of about eight glycerophosphate units, two of which had a 2-acetamido-2-deoxy--d-galactopyranosyl substituent and one, a 3-O-methyl--d-galactopyranosyl-(1 3)-2-acetamido-2-deoxy--d-galactopyranosyl residue at C2 of glycerol. The structure of the polymer was identified by chemical analysis and 13C-NMR spectroscopy. The teichoic acid contained 3-O-methyl-d-galactose (madurose) — the first ever finding of this compound within a teichoic acid. The nonpeptidoglycan amino acids made up some 30% of the cell wall's dry weight, about a quarter of the amino acids being removable with sodium dodecyl sulfate. Further treatment of the cell walls with LiCl and guanidine hydrochloride caused only a small loss of the amino acids and slight changes in their molar ratio.Abbreviations Gro glycerol - GroP monophosphate glycerol - GroP2 diphosphate glycerol - Gro2P -monophosphate glycerol - PTA phosphorus of teichoic acids - PNA phosphorus of nucleic acids - TA teichoic acid |
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Keywords: | Aetinomadura Cell walls Teichoic acids Peptidoglycan Protein Madurose |
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