Degradation of tetracyanonickelate (II) by Cryptococcus humicolus MCN2 |
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Authors: | Kwon Hyouk K Woo Seung H Park Jong M |
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Institution: | Department of Chemical Engineering, School of Environmental Science and Engineering, Pohang University of Science and Technology, San 31, Pohang 790-784, South Korea. |
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Abstract: | A new yeast strain capable of degrading free and metallocyanides was isolated from coke-plant wastewater. The isolated strain designated MCN2 was identified as Cryptococcus humicolus by 26S rDNA sequencing and phylogenetic analysis. During growth of the isolate with KCN as a sole nitrogen source, formamide and formic acid were found as transient intermediates by (13)C]nuclear magnetic resonance analysis and ammonia accumulated as a final product in the culture medium. The strain MCN2 could degrade high concentrations of tetracyanonickelate (II) (K(2)Ni(CN)(4), TCN) up to 65 mM CN within 60 h when a sufficient amount of glucose was supplied as a carbon source. The maximal degradation rate of TCN was 2.5 mM CN h(-1) at the initial concentration of 51 mM CN. |
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Keywords: | Biodegradation Cyanide Tetracyanonickelate Cryptococcus humicolus |
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