Characterization of Virion RNAs of Southern Bean Mosaic Virus by Electron Microscopy |
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| Authors: | O. P. Sehgal S. El-Hassan K. G. Murti C. H. Hsu |
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| Affiliation: | Department of Plant Pathology, University of Missouri, Columbia, Missouri, U.S.A. and Division of Virology, St. Jude Children's Research Hospital, Memphis, Tennessee, U.S.A. |
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| Abstract: | Electron microscopy of denatured RNA of southern bean mosaic virus (SBMV) shows two principal linear components, 0.31 ± 0.08 μm (subgenomic RNAs, MW 0.51 × 106) and 0.80 ± 0.17 μm (genomic RNA, MW 1.3 × 106 25S). Nondenatured RNA (～ 32S) from heat-inactivated virions measure 1.0 ± 0.20 μm (MW 1.64 × 106) but is poorly-infectious. Upon denaturation the 325 RNA disaggregates into components of lengths typical of the genomic and subgenornic RNAs and infectivity is restored. |
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