Purification and characterisation of an alkaline protease used in tannery industry from Bacillus licheniformis |
| |
| Authors: | Xue-Ming Tang FM Lakay Wei Shen Wei-Lan Shao Hui-Ying Fang BA Prior Zheng-Xiang Wang Jian Zhuge |
| |
| Institution: | The Key Laboratory of Industrial Biotechnology, Ministry of Education, Research Center of Industrial Microbiology, Southern Yangtze University, Wuxi 214036, PR China. txm@sun.ac.za |
| |
| Abstract: | An extracellular alkaline protease produced by Bacillus licheniformis AP-1 was purified 76-fold, yielding a single 28 kDa band on SDS-PAGE. It was optimally active at pH 11 and at 60 degrees C (assayed over 10 min). The protease was completely inhibited by phenylmethylsulfonyl fluoride and diodopropyl fluorophosphate, with little increase upon Ca2+ and Mg2+ addition. |
| |
| Keywords: | alkaline protease Bacillus licheniformis characterisation purification |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
