DNA stretching on functionalized gold surfaces. |
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| Authors: | R M Zimmermann and E C Cox |
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| Affiliation: | Department of Molecular Biology, Princeton University, NJ 08544. |
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| Abstract: | We describe a method for anchoring bacteriophage lambda DNA by one end to gold by Au-biotin-streptavidin-biotin-DNA bonds. DNA anchored to a microfabricated Au line could be aligned and stretched in flow and electric fields. The anchor was shown to resist a force of at least 11 pN, a linkage strong enough to allow DNA molecules of chromosome size to be stretched and aligned. |
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