| 太子参超低温脱毒及规模化组培育苗技术 |
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| 引用本文： | 戴军,;姚厚军,;张九玲,;韩邦兴,;陈乃富. 太子参超低温脱毒及规模化组培育苗技术[J]. 生物学杂志, 2014, 0(3): 84-87 |
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| 作者姓名： | 戴军, 姚厚军, 张九玲, 韩邦兴, 陈乃富 |
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| 作者单位： | [1]皖西学院生物与制药工程学院中药研究与开发工程技术研究中心,安徽六安237012; [2]植物细胞工程安徽省工程技术研究中心,安徽六安237012 |
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| 基金项目： | 中央财政国家林业局林业推广项目（[2012]TQ06）; 安徽省高等学校省级自然科学基金项目（KJ2011A270、KJ2013A265）; 安徽省六安市定向委托项目（2013LWA008） |
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| 摘 要： | 以太子参茎尖为外植体,采用超低温去除病毒方法,通过组织培养研究了芽增殖诱导、丛生芽诱导、生根壮苗诱导的适合条件,以期形成太子参规模化育苗技术。结果表明：超低温处理1 h后,太子参脱毒率可达90%以上;规模化组培育苗最适宜配方为初代培养基为改良MS＋2.5 mg/L 6-BA＋0.5 mg/L IAA继代增殖培养基为改良MS＋1.2 mg/L KT＋0.5 mg/L NAA,壮苗生根培养基为改良MS＋0.2 mg/L KT＋1.5 mg/L DA-6＋10%蛋白粉。
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| 关 键 词： | 太子参 超低温 脱毒 规模化育苗 |
Super low temperature seedling-raising technology of virus-elimination scale tissue culture of Pseudostellaria heterophylla |
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| Affiliation: | DAI Jun,YAO Hou-jun,ZHANG Jiu-ling,HAN Bang-xing,CHEN Nai-fu( 1. College of Biological and Pharmaceutical Engineering, West Anhui University, Engineering Technology Research Center of Research and Development of Traditional Chinese Medicine, Lu＇an 237012; 2. Engineering Technology Research Center of Plant Cell Engineering, Lu＇an 237012, China) |
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| Abstract: | The shoot tips of Pseudostellaria heterophylla as explants in vitro culture was conducted for adventitious bud induction,bud multiplication and root induction under super low temperature treatment,in order to form a large-scale breeding technology. The results showed that with super low temperature treatment for 1 hours,radix Pseudostellariae virus-free rate might reach above 90%. The most suitable initial culture medium in the fast reproduction system was MS ＋ 2. 5 mg /L 6- BA ＋ 0. 5 mg /L IAA,and the subculture medium was MS ＋ 1. 2 mg /L KT ＋ 0. 5 mg /L NAA. The highest proliferation rate was 12. 5. The rooting medium was MS ＋ 0. 2 mg /L KT ＋1. 5 mg /L DA- 6 ＋ 10% protein powder. |
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| Keywords: | Pseudostellaria heterophylla super low temperature virus elimination industrial seedling production |
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