Immunoprecipitation of a pertussis toxin substrate of the Go family from rat islets of Langerhans |
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Authors: | Nicholas S Berrow Roger D Hurst Susan L F Chan Noel G Morgan |
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Institution: | (1) Cellular Pharmacology Group, Department of Biological Sciences, Keele University, ST5 5BG Keele, Staffs, UK;(2) Department of Medicine, University of Toronto, M5S 1A8 Toronto, Ontario, Canada |
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Abstract: | Rat islets express a pertussis toxin sensitive G-protein involved in receptor-mediated inhibition of insulin secretion. This
has been assumed previously to represent “Gi” which couples inhibitory receptors to adenylate cyclase. Incubation of islet G-proteins with32P-NAD and pertussis toxin resulted in the labelling of a band of molecular weight 40,000. This band was very broad and did
not allow resolution of individual components. Incubation of the radiolabelled proteins with an anti-Go antiserum resulted in specific immunoprecipitation of a32P-labelled band. These results demonstrate that the complement of pertussis toxin sensitive G-proteins in rat islets includes
Go. |
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Keywords: | Insulin secretion pancreatic B-cell G-protein adenylate cyclase |
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