ShiftDetector: detection of shift mutations |
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Authors: | Seroussi Eyal Ron Micha Kedra Darek |
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Affiliation: | Institute of Animal Science, Volcani Center, Bet-Dagan 50250, Israel. seroussi@agri.huji.ac.il |
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Abstract: | MOTIVATION: Sequencing of a bi-allelic PCR product, which contains an allele with a deletion/insertion mutation results in a superimposed tracefile following the site of this shift mutation. A trace file of this type hampers the use of current computer programs for base calling. ShiftDetector analyses a sequencing trace file in order to discover if it is a superimposed sequence of two molecules that differ in a shift mutation of 1 to 25 bases. The program calculates a probability score for the existence of such a shift and reconstructs the sequence of the original molecule. AVAILABILITY: ShiftDetector is available from http://cowry.agri.huji.ac.il |
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