Sperm characteristics and heterologous in vitro fertilisation capacity of Iberian ibex (Capra pyrenaica) epididymal sperm,frozen in the presence of the enzymatic antioxidant catalase |
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Authors: | J López-Saucedo MT Paramio R Fierro D Izquierdo MG Catalá MA Coloma A Toledano-Díaz A López-Sebastián J Santiago-Moreno |
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Institution: | 1. Fertility Center Cancún, Cancún, Mexico;2. Departamento de Ciencia Animal y de los Alimentos, Facultad de Veterinaria, Universidad Autónoma de Barcelona, 08193 Bellaterra, Barcelona, Spain;3. Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, 09340 Mexico City, Mexico;4. Departamento de Reproducción Animal, INIA, 28040 Madrid, Spain |
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Abstract: | The aim of this work was to evaluate the protective effect of catalase (CAT) on frozen/thawed ibex epididymal sperm recovered post mortem, and to detect any harmful effect this might have on sperm fertilisation capacity. Epididymal spermatozoa were diluted using a Tris–citric acid–glucose medium (TCG) composed of 3.8% Tris (w/v), 2.2% citric acid (w/v), 0.6% glucose (w/v), 5% glycerol (v/v), and 6% egg yolk (v/v). Sperm masses from the right epididymis were diluted with TCG medium, while those from the left were diluted with TCG medium supplemented with 200 IU/mL CAT. Heterologous in vitro fertilisation (IVF) was used to assess the fertilisation capacity of this sperm. The addition of CAT to the extender did not improve frozen/thawed sperm variables. Moreover, a reduced fertilisation capacity was detected: sperm diluted with TCG provided 25.5% 2PN zygotes, while just 13.2% was recorded for that diluted with TCG-CAT (P < 0.01). The percentage of cleaved embryos at 48 hpi was higher (P < 0.01) with the TCG sperm than with the TCG-CAT sperm (16.7% vs. 7.6%). The use of 200 IU/mL CAT as an additive cannot, therefore, be recommended for the preservation of ibex epididymal sperm. Other antioxidants should, however, be tested in both this and related wild mountain ungulates. |
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Keywords: | Catalase Heterologous in vitro fertilisation Epididymal sperm Ibex Frozen-thawed semen |
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