Supplementing culture and vitrification-warming media with l-ascorbic acid enhances survival rates and redox status of IVP porcine blastocysts via induction of GPX1 and SOD1 expression |
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Authors: | Miriam Castillo-Martí n,Sergi Bonet,Roser Morató ,Marc Yeste |
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Affiliation: | 1. Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, E-17071 Girona, Spain;2. Unit of Animal Reproduction, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, E-08193 Bellaterra (Barcelona), Spain |
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Abstract: | |
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Keywords: | AC, l-ascorbic acid CNT, control GPX1, glutathione peroxidase 1 IVC, in vitro culture IVC-CNT, blastocysts cultured without AC IVC-AC, blastocysts cultured with AC IVP, in vitro produced SOD1, superoxide dismutase 1 SOD2, superoxide dismutase 2 TCN, total cell number VW, vitrification-warming VW-CNT, blastocysts vitrified-warmed without AC VW-AC, blastocysts vitrified-warmed with AC |
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