Selective inhibition of transition from sexual agglutination to zygote formation by ethyl N-phenylcarbamate in Saccharomyces cerevisiae |
| |
Authors: | Satoshi Hasegawa Naohiko Yanagishima |
| |
Affiliation: | (1) Biological Institute, Faculty of Science, Nagoya University, 464 Nagoya, Japan |
| |
Abstract: | Effects of ethyl N-phenylcarbamate (EPC) on the mating reaction of Saccharomyces cerevisiae were studied, with special attention on the effect on the pheromone action. EPC inhibited zygote formation at a concentration which promoted induction of sexual agglutinability. EPC enhanced agglutinability induction by pheromone, but inhibited -pheromone-induced formation of large pearshaped cells in a mating type. The enhancement of agglutinability induction was accompanied with increased production of a agglutination substance and inhibition of pheromone inactivation. EPC arrested the cell cycle of a cells probably in the step controlled by CDC19, CDC35, cAMP etc., just before the step controlled by CDC28, pheromone etc.Abbreviations EPC Ethyl N-phenylcarbamate - PBS 0.01 M phosphate buffer solution, pH 5.5 - SPB spindle pole body |
| |
Keywords: | Agglutination substance /content/x142jg127084183n/xxlarge945.gif" alt=" agr" align=" BASELINE" BORDER=" 0" > Pheromone Cell cycle Ethyl N-phenylcarbamate Mating reaction Microtubules Saccharomyces cerevisiae |
本文献已被 SpringerLink 等数据库收录! |
|