Recognition of glycoconjugates by Helicobacter pylori. Comparison of two sialic acid-dependent specificities based on haemagglutination and binding to human erythrocyte glycoconjugates |
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Authors: | Halina Miller-Podraza Jorgen Bergstrom Maan Abul Milh Karl-Anders Karlsson |
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Institution: | (1) Department of Medical Biochemistry, Goteborg University, Medicinaregatan 9A, S-413 90 Goteborg, Sweden |
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Abstract: | Helicobacter pylori expresses separate binding characteristics depending on growth conditions, as documented by binding to
human erythrocyte glycoconjugates. Cells grown in Ham's F12 liquid medium exhibited a selective sialic acid-dependent binding
to polyglycosylceramides, PGCs (Miller-Podraza et al. (1996) Glycoconjugate J 13:453–60). There was no binding to traditional
sialylated glycoconjugates like shorter-chain gangliosides, glycophorin or fetuin. However, cells grown on Brucella agar bound
both to PGCs and other sialylated glycoconjugates. Fetuin was an effective inhibitor of haemagglutination caused by agar-grown
cells, but had no or a very weak inhibitory effect on haemagglutination by F12-grown bacteria. PGCs were strong inhibitors
in both cases, while asialofetuin was completely ineffective. The results indicate that H. pylori is able to express two separate
sialic acid-dependent specificities, one represented by binding to fetuin, as described before, and another represented by
a selective binding to PGCs. Abbreviations: PGCs, polyglycosylceramides; TLC, thin-layer chromatography; SDS PAGE, sodium
dodecylsulfate polyacrylamide gel electrophoresis; BSA, bovine serum albumin; C, chloroform; M, methanol. The carbohydrate
and glycosphingolipid nomenclatures are according to recommendations of IUPAC-IUB Commission on Biochemical Nomenclature (Lipids
(1977) 12:455–68; J Biol Chem (1982) 257:3347–51 and J Biol Chem (1987) 262:13–18).
This revised version was published online in November 2006 with corrections to the Cover Date. |
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Keywords: | Helicobacter pylori human erythrocytes sialic acid polyglycosylceramides |
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