火炬松成熟合子胚培养直接器官发生和植株再生

基因型Ｈｂ，Ｍａ和Ｍｃ的火炬松成熟合子胚在附加１．０ｍｇ／ＬＮＡＡ，４．０ｍｇ／ＬＢＡ，５００ｍｇ／ＬＬＨ和５００ｍｇ／Ｌ谷氨酰胺的ＴＥ培养基上培养１２周后，在子叶和胚轴部位形成不定芽原基。然后将合子胚转移到附加０．５ｍｇ／／ＬＮＡＡ，０．０５ｍｇ／ＬＩＢＡ，２ｍｇ／ＬＢＡ，５００ｍｇ／ＬＬＨ和５００ｍｇ／Ｌ谷氨酰胺的ＴＥ不定芽分化培养基上，６周后分化产生大量不定芽，３种基因型中，Ｈｂ的直接不定芽

DIRECT ORGANOGENESIS AND PLANT REGENERATION FROM MATURE ZYGOTIC EMBRYOS OF LOBLOLLY PINE

Hb, Ma and Mc genotype mature zygotic embryos of loblolly pine were cultured on TE medium containing 1.0 mg/L NAA, 4.0 mg/L BA, 500 mg/L LH and 500 mg/L glutamine, and primary adventitious buds were formed on cotyledons and hypocotyl in 12 weeks. Zygotic embryos with primary adventitious buds were transferred to TE differentiation medium with 0.5 mg/L NAA, 0.5 mg/L IBA, 2 mg/L BA, 500 mg/L LH and 500 mg/L glutamine. After 6 weeks a lot of adventitious buds appeared. Among 3 genotypes, differentiation frequency of Hb genotype adventitious bud was 58.2% the highest. Direct adventitious buds which were cultured or elongation medium for 6 weeks were transferred to TE rooting medium supplemented with 0.1 mg/L IBA, 1.0 mg/L BA, 0.5 mg/L GA 3, and the highest rooting frequency of adventitious bud was 46% in 6 weeks.