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| 氟对成骨细胞样细胞胞内钙和钙通道电流的影响 | |
| 引用本文： | Hua K,Zhao H,Huang M,Li GS. 氟对成骨细胞样细胞胞内钙和钙通道电流的影响[J]. 中国应用生理学杂志, 2003, 19(2): 179-181 |
| 作者姓名： | Hua K Zhao H Huang M Li GS |
| 作者单位： | 1. 吉林大学再生医学研究所病理研究室,吉林,长春,130021 2. 吉林大学中日联谊医院中心研究室,吉林,长春,130031 3. 吉林大学白求恩医学部生理教研室,吉林,长春,130021 |
| 基金项目： | 国家自然科学基金资助项目 ( 39730 390 ) |
| 摘 要： | 目的 :观察氟 (Fluoride,F-)对培养的成骨细胞样细胞胞内钙和钙通道电流的影响。方法 :应用Fura 2负载的成骨细胞样细胞 ,通过紫外荧光分光光度仪测定细胞内游离钙的浓度 ,同时应用全细胞膜片钳技术记录成骨细胞样细胞钙通道的变化。结果 :氟可引起成骨细胞样细胞胞内钙增高 ,尤以 10 0ng/mlF-最为明显 ,与对照组比较差异显著 (P <0 .0 5 )。应用全细胞膜片钳技术发现 2 5ng/mlF-即可引起成骨细胞样细胞钙通道开放 ,随着染F-剂量的增加 ,Ca2 +通道电流的幅值增大 (P <0 .0 1) ,且F-对Ca2 +通道电流的兴奋作用呈剂量依赖性 (r =0 .9914)。结论 :F-可引起成骨细胞样细胞钙通道开放 ,从而导致细胞胞内钙浓度的增加。 |
| 关 键 词： | 氟化物 成骨细胞 钙 膜片钳 通道 电流 氟骨症 |
| 文章编号： | 1000-6834(2003)02-0179-03 |
| 修稿时间： | 2002-06-14 |
Effects of fluoride on ionized calcium and calcium channel in osteoblast-like cell in vitro |
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| Hua Kun,Zhao Hong,Huang Min,Li Guang-sheng. Effects of fluoride on ionized calcium and calcium channel in osteoblast-like cell in vitro[J]. Chinese journal of applied physiology, 2003, 19(2): 179-181 | |
| Authors: | Hua Kun Zhao Hong Huang Min Li Guang-sheng |
| Affiliation: | HUA Kun1,ZHAO Hong2,HUANG Min3,LI Guang-sheng1 |
| Abstract: | Aim: To study the effects of fluoride (F-) on ionized calcium ( i) and calcium channel in osteoblast-like cell(OBLs). Methods: Invistigating i was by fluorospectrophotometry and recording the calcium currents of OBLs was using whole patch-clamp technique. Results: The addition of fluoride to the medium made a rapid and significant increase in free i , especially in 100 ng F-/ml group. There were significances between control group and 50,100 ng F-/ml groups. And 25 ng/ml F- could stimulate OBLs calcium channel open. Compared with control group, fluoride could significantly increase the amplitudes of calcium currents (P<0.01), furthermore the activation was in dose-dependent manner. Conclusion: Fluoride can make calcium channel open and then increase the concentrations of i. |
| Keywords: | fluoride osteoblast calcium patchclamp technique |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |