| 转基因动物乳腺暂时性表达系统的建立 |
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| 引用本文： | 卢一凡,邓继先,肖成祖,马清钧. 转基因动物乳腺暂时性表达系统的建立[J]. 生物技术通讯, 1998, 0(2) |
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| 作者姓名： | 卢一凡 邓继先 肖成祖 马清钧 |
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| 作者单位： | 军事医学科学院生物工程研究所，军事医学科学院生物工程研究所，军事医学科学院生物工程研究所，军事医学科学院生物工程研究所 北京 100071，北京 100071，北京 100071，北京 100071 |
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| 摘 要： | 以乳清酸蛋白(WAP)基因5′区为调控序列,人基因组G-CSF基因为目的片段,同时将WAP基因第一内含子插入G-CSF基因5′端,构建成转基因动物乳腺表达载体。将其直接注射到小鼠乳腺,在泌乳期表达出人G-CSF。表明乳腺直接注射质粒的方法可以做为暂时性的一种表达系统,同时也表明内含子对表达有一定的作用。
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| 关 键 词： | 直接注射 乳腺暂时表达 内含子 |
A convenient transient system of mammary gland expression in transgenic animal |
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| Lu Yifan,Deng Jixian,Xiao Chengzu,Ma Qingjun. A convenient transient system of mammary gland expression in transgenic animal[J]. Letters in Biotechnology, 1998, 0(2) |
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| Authors: | Lu Yifan Deng Jixian Xiao Chengzu Ma Qingjun |
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| Abstract: | The expression plasmid pINGG that WAP gene 5' control region directs Chinese genomic DNA and that WAP first intron was inserted to G-CSF 5' region was constructed. This plasmid was injected to mammary gland of mice, and human G-CSF was obtained in milk of mice. Expression level got 15-32 ng/ml. This result showed that plasmid constructed could be used to transgenic animal, meanwhile, mammary gland injection method could provide us a convenient transient system to confirm vector's validity, and also showed that nitron has certain effect on expression. |
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| Keywords: | mammary transient expression injection inlron |
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