Bifidobacteria as indicators of faecal contamination along a sheep meat production chain |
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Authors: | Delcenserie V Loncaric D Bonaparte C Upmann M China B Daube G Gavini F |
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Institution: | Food Sciences Department, Faculty of Veterinary Medicine, University of Liège, Liege, Belgium; Institute of Meat Hygiene, Meat Technology and Food Science, University of Veterinary Medicine Vienna, Wien, Austria; Département Microbiologie et Chaîne Alimentaire, Institut National de la Recherche Agronomique, Villeneuve d'Ascq, France |
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Abstract: | Aims: The potential use of bifidobacteria as indicators for faecal contamination was studied along a sheep meat production and processing chain. The levels of bifidobacteria were compared with those of Escherichia coli . Total viable counts were followed along the chain (244 samples). Methods and Results: Forty-three per cent of the samples contained bifidobacteria, of which 15% were solely detected using a PCR method based on the hsp60 gene and not by a culture-based method. Bifidobacteria were detected in only three of nine sheep faeces samples using one or the other method. However, carcasses (types C and E) were highly contaminated. These sample types (30% and 28%, respectively) were positive for bifidobacteria and negative for E. coli . The species Bifidobacterium pseudolongum and Bif. thermophilum , isolated from faecal samples, were predominant. Bifidobacterium choerinum were found in C, D, E and F sample types. Conclusions: Bifidobacteria were shown more efficient than E. coli in carcasses samples. The presence of Bif. choerinum suggested a faecal pork contamination. Significance and Impact of the Study: Detection and identification of bifidobacteria, in correlation with E. coli counting, should improve hygiene quality of mutton processing chains. |
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Keywords: | Bifidobacterium choerinum Bifidobacterium pseudolongum Bifidobacterium thermophilum DNA–DNA hybridization faecal indicators hsp60 gene meat mupirocin PCR sheep slaughter |
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