胆囊癌组织中IL-8表达及TAM计数的临床病理意义

目的:检测胆囊腺癌组织中白细胞介素-8(IL-8)mRNA的表达、肿瘤相关巨噬细胞(TAM)计数并探讨其临床病理意义。方法:收集中南大学湘雅二医院及湖南省人民医院近五年胆囊腺癌手术切除标本36例及慢性胆囊炎手术切除标本10例,采用原位分子杂交方法检测IL-8表达,ABC免疫组化法进行TAM计数。比较胆囊腺癌和慢性胆囊炎标本组织中IL-8 mRNA表达和TAM计数的差异,并分析其与胆囊腺癌临床病理特征之间的关系。结果:胆囊腺癌组织中IL-8 mRNA表达阳性率及其评分均明显高于慢性胆囊炎(P0.01),IL-8 mRNA表达阳性率及其评分与其侵犯胆总管及发生淋巴结转移显著相关(P0.05)。胆囊腺癌组织中TAM计数(24.89±0.84)明显高于慢性胆囊炎组织(16.19±0.66),差异有统计学意义(P0.01);侵犯胆总管、肝脏及发生淋巴结转移的胆囊腺癌组织中TAM计数高于未侵犯胆总管、肝脏及发生淋巴结转移的胆囊腺癌组织TAM计数,其中侵犯胆总管和发生淋巴结转移之间差异显著(P0.01)。IL-8 mRNA阳性病例的TAM计数均明显高于阴性病例(P0.01),TAM计数与IL-8 mRNA评分间也存在显著正相关(r=0.748,P0.001)。结论:IL-8和TAM计数与胆囊癌的发生和发展密切相关,IL-8可能在促进TAM向胆囊癌组织迁移浸润中起作用。

Clinicopathological Significance of IL-8 Expression and TAMCount in Gallbladder Carcinoma

Objective:To investigate the expression of IL-8 mRNA and Tumor associated macrophages(TAM)count in gallbladder carcinoma tissues and their clinical significance.Methods:36 cases of gallbladder carcinoma operation samples and 10 cases of Chronic cholecystitis operation samples were collected from Hunan province Ren min Hospital and Second Xiangya Hospital. The expression of IL-8 was detected by in situ hybridization method, TAMcount was determined by ABC immunohistochemical method. The difference of IL-8 mRNA expression and TAM count were compared between gallbladder carcinoma and Chronic cholecystitis, and their relationship with clinicopathological features of gallbladder carcinoma were analyzed.Results:The positive rate and score of IL-8mRNA expression in gallbladder carcinoma tissues were significantly higher than those of the chronic cholecystitis tissues (P<0.01). The positive rate and score of IL-8mRNA expression in gallbladder carcinoma tissues were significantly related to the infringement of common bile duct and the incidence of lymph node metastasis. TAMcount of Gallbladder adenocarcinoma (24.89± 0.84) was significantly higher than that of the chronic cholecystitis tissues(16.19± 0.66)(P<0.01). gallbladder adenocarcinoma TAMcount with violations of common bile duct, the liver and lymph node metastasis were higher than those without violations of common bile duct, liver and lymph node metastasis with significant differences (P<0.01). TAM count of IL-8 mRNA positive cases were significantly higher than those in IL-8 mRNA negative cases (P<0.01).Conclusion:IL-8 and TAMcount were closely related to the occurrence and development of gallbladder carcinoma, and IL-8 may promote TAMinfiltrating to gallbladder carcinoma tissue.