GDF15基因在卵巢上皮性癌组织中的表达及其临床意义

目的:探讨生长分化因子GDF15(Growth Differentiation Factor 15)基因在卵巢上皮性癌组织中的表达及其与铂类耐药的相关性。方法:应用免疫组化、western blot、RT-PCR等方法对80例原发性卵巢癌组织和卵巢癌顺铂敏感/耐药株A2780和CP70、SKOV3和SKOV3/DDP中生长分化因子GDF15表达水平进行测定。结果:生长分化因子GDF15的表达强度与卵巢癌铂类耐药性显著相关。在卵巢癌顺铂耐药株CP70、SKOV3/DDP中GDF15表达水平较顺铂敏感株A2780、SKOV3明显增高。结论:GDF15表达水平与卵巢癌发生发展及铂类耐药相关,对于卵巢癌患者早期筛选、预测预后具有一定的临床指导价值。

The Clinical Significance of GDF15 Expression in Human Ovarian Cancer

Objective:To study the relationship of the Growth Differentiation Factor 15 (GDF15) expression in human ovarian cancer and the resistant of human epithelial ovarian cancer to cisplatin.Methods:Expression and location of GDF15 protein was detected in specimens of 80 human epithelial ovarian cancer patients and 4 human ovarian cancer cell lines.Results:cytoplasmic localization of GDF15 positive staining could be observed in human epithelial ovarian cancer. The intensity of GDF15 protein was significantly correlated with FIGO, differentiation, malignant ascites, lymphatic metastasis, and platinum resistance in ovarian cancer. GDF15 mRNA levels in cisplatin resistant ovarian cancer cell lines CP70 and SKOV3/DDP increased significantly compared to those in cisplatin sensitive cell lines A2780 and SKOV3. Treated with cisplatin, GDF15 protein levels were up-regulated in both A2780 and CP70 cells.Conclusion:GDF15 expression appear to differ between platinum sensitive and platinum resistant ovarian cancers. Thus GDF15 expression may be useful biomarker for early screening and predicting prognosis in ovarian cancer patients.