血管内皮生长因子(VEGF)乳液法电纺纤维膜的体外研究

目的:研究担载血管内皮生长因子(VEGF)的乳液法电纺纤维膜的亲水性能、外观形态和机械性能,纤维膜中VEGF的包封率和体外释放动力学,为评价其能否应用于血管再生领域的研究奠定基础。方法:将VEGF水溶液通过W/O乳液法制备成缓释VEGF的生物可降解的丙交酯-乙交酯共聚物(PLGA)静电纺丝纤维膜,对该纤维膜的接触角、外观形态、机械性能进行表征,Elisa法测定该纤维膜的体外14天的释放行为,分别观察纤维膜释放0天、7天、14天后的电镜图。结果:加入VEGF后,纤维膜的接触角由140.0°减小到136.1°,亲水性增强,具有类似细胞外基质(ECMs)网状结构和良好的力学性能,纤维膜第1天的突释不超过载药量的50%,电镜图下显示纤维膜释放1周时纤维发生断裂。结论:通过乳液法制备的担载VEGF的电纺纤维膜具有良好的物理性能,能够持续缓释VEGF,可作为血管再生的组织工程支架进行深入研究。

Study on the Vascular Endothelial Growth Factor (VEGF) emulsion Electrospun fiber Membrane in Vitro

Objective:To investigate the hydrophilic performance, morphology and mechanical properties of emulsion electrospun fiber membrane loading vascular endothelial growth factor (VEGF), the coating rate and release kinetics of VEGF in vitro in the fiber membrane, and evaluate whether it could be applied in the research of angiogenesis.Methods:VEGF aqueous solution was prepared into a VEGF sustained-release PLGA electrospun fiber membrane by W/O emulsion method. The contact Angle, exterior morphology, and mechanical properties of the fiber membrane were characterized. Elisa method was to detect the release behavior of the fiber membrane in vitro. The fiber membrane SEM0 day, 7 days, and 14 days after release were observed respectively.Results:After adding VEGF, the contact Angle of fiber membrane reduced from 140.0° to 136.1° , its hydrophilicity enhanced, the fiber membrane had similar extracellular matrix (ECMs) mesh structure and good mechanical properties, initial burst release fiber membrane was no more than 50% drug loading capacity on the first day, fiber membrane had a break under SEMin a week.Conclusion:VEGF emulsion electrospun fiber membrane had good physical properties, continuous sustained release of VEGF, which could be used as angiogenesis tissue engineering scaffolds for further research.