CCNB2在结直肠癌中的表达及临床意义

目的:探讨细胞周期蛋白B2(Cyclin B2,CCNB2)在结直肠癌组织中的表达及其临床意义。方法:选择45对结直肠癌组织及癌旁正常结直肠组织样本,分别采用实时定量PCR(qRT-PCR)方法和免疫组织化学技术检测CCNB2的mRNA和蛋白表达,并进一步分析CCNB2的表达与结直肠癌临床病理特征之间的关系。结果:结直肠癌组织中CCNB2 mRNA的表达显著高于癌旁正常结直肠组织,差异有统计学意义(P0.001),且CCNB2的mRNA表达与结直肠癌的肿瘤大小、浸润深度及TNM分期显著相关(P0.05),与年龄、性别、肿瘤位置、分化程度、脉管神经浸润、淋巴结转移和远处转移均无关(P0.05)。45例结直肠癌标本中39例表达(+~+++),6例表达(-)。CCNB2蛋白主要表达于结直肠癌细胞质中,少量见于细胞核。结直肠癌组织中CCNB2蛋白的阳性表达率为86.7%,显著高于癌旁正常结直肠组织,并与患者的性别、年龄、分化程度和肿瘤转移均无显著相关性(P0.05),但与肿瘤分期、浸润程度均显著相关(P0.05)。结论:CCNB2在结直肠癌中呈异常高表达,且与结直肠癌的发生发展相关,有望作为结直肠癌的诊断和预后预测参考指标。

Expression and Clinical Significance of Cyclin B2 in Colorectal Cancer

Objective:To investigate the expression and clinical significance of Cyclin B2 (CCNB2) in colorectal cancer (CRC).Methods:The expression of CCNB2 in 45 pairs of CRC tissues and para-adjacent normal tissues were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry. The relationship between the expression of CCNB2 and clinicopathologic characteristics was analyzed by SPSS 17.0.Results:The expression of CCNB2 mRNA in CRC tissue was significantly higher than that of the para-adjacent non-malignant tissues (P<0.001). It was significantly correlated with the tumor size, greater tumor depth, TNMstage and lymph node metastasis of CRC (P<0.05), but had no correlation with the age or gender of patients, tumor location, differentiation, venous and nervous invasion, lymph nodes metastasis and distant metastasis (P>0.05). The immuno-activity was detected mainly in the cytoplasm of colorectal cancer cells. The positive rate of CCNB2 protein expression in CRC tissues was 86.7%, markedly higher than that of the para-adjacent non-malignant tissues(P<0.001), and related to the tumor stage and invasion(P<0.05), but it had no correlation with the age or gender of patients, differentiation, metastasis (P>0.05).Conclusion:Overexpression of CCNB2 in colorectal cancer could be associated with the occurrence and progress of CRC and expected to be the diagnositic and prognostic indicator of CRC.