埃兹蛋白的表达与非小细胞肺癌转移的关系研究

目的:探究埃兹蛋白(Ezrin)的表达与非小细胞肺癌转移的关系。方法:通过免疫组化检测Ezrin在有无转移的非小细胞肺癌组织中的表达差异,通过细胞免疫组化、western-blot、RT-PCR检测Ezrin在不同转移潜能肺癌细胞系中的表达差异,通过transwell考察Ezrin对不同转移潜能癌细胞侵袭和迁移能力的影响。结果:Ezrin蛋白在有转移的非小细胞肺癌组织中的表达水平明显高于无转移的肺癌组织,在高转移潜能肺癌细胞系中的表达高于低转移潜能细胞系,受抑制时会削弱高转移潜能肺癌细胞的迁移和侵袭能力,过表达时会增强低转移潜能肺癌细胞的侵袭和迁移能力。结论:Ezrin可能在非小细胞肺癌及其转移中发挥重要作用。

The Study of Relationship between Ezrin Protein Expression and NSCLCMetastasis

Objective:To investigate the relationship between Ezrin protein expression and Non-small cell lung cancer (NSCLC)metastasis.Methods:The different expressions of Ezrin in NSCLC tissues with and without metastasis were detected byimmunohistochemistry. The different expressions of Ezrin in Non-small cell lung cancer cells with different metastasis potential werestudied by immunohistochemistry, western-blot and RT-PCR. The effects of Ezrin to invasion and migration abilities of cancer cells withdifferent metastasis potential were studied by transwell.Results:The expression of Ezrin protein in Non-small cell lung cancer tissueswith metastasis was higher than that without metastasis. The expression of Ezrin in lung cancer cells with high metastasis potential washigher than that with low metastasis potential. The invasion and migration abilities of cancer cells with high metastasis potentialweakened when Ezrin inhibited, while the abilities of cancer cells with low metastasis potential strengthened when Ezrin overexpressed.Conclusion:Ezrin may play an important role in NSCLC and its metastasis.